Journal: Pathogens and Immunity

Article Title: Replication and Injury Associated With SARS-CoV-2 in Cultured Hepatocytes

doi: 10.20411/pai.v8i2.648

Figure Lengend Snippet: Caspase-3 expression and quantification. Cell lines, 70%-80% confluent, were preincubated with 0.5µM and 5µM remdesivir for 24 hours in a 12.5 mm polyester membrane transwell plate. Following incubation, the cells were infected with SARS-CoV-2 at MOI of 0.1 for 1 hour, washed with PBS (3X), and replaced with complete growth media. Infected cells were further incubated for 3 days before being stained for apoptotic marker caspase-3 (A) and quantified (B). Virus only was used as positive control and no virus as negative control. (B) The fluorescent signal was quantified using ImageJ software. Statistical analysis was done using GraphPad Prism (version 9) software, using one-way ANOVA. * P = 0.0146, ** P = 0.0024, **** P = <0.0001. Fluorescent signal was captured using Zeiss LSM710 LIVE Duo confocal microscope. Bar indicates 20µm. n=3

Article Snippet: Quantification and statistical analysis on the expressed caspase-3 antibody indicated that cell death was reduced with the antiviral drug remdesivir at 0.5µM concentration in all cell lines.

Techniques: Expressing, Membrane, Incubation, Infection, Staining, Marker, Virus, Positive Control, Negative Control, Software, Microscopy